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Course Unit Title Course Unit Code Type of Course Unit Level of Course Unit Year of Study Semester ECTS Credits
Molecular Methods In Microbiology MKB712 Compulsory Doctorate degree 1 Spring 5

Name of Lecturer(s)

Prof. Dr. Fetiye KOLAYLI

Learning Outcomes of the Course Unit

1) Know the structure of nucleic acids
2) Has knowledge about the genetic structures of microorganisms
3) Knows nucleic acid amplification methods
4) Knows typing methods of microorganisms

Program Competencies-Learning Outcomes Relation

  Program Competencies
1 2 3 4 5 6 7 8
Learning Outcomes
1 Low Middle Middle No relation No relation No relation No relation No relation
2 No relation No relation No relation No relation No relation No relation No relation No relation
3 No relation No relation No relation No relation No relation No relation No relation No relation
4 No relation No relation No relation No relation No relation No relation No relation No relation

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Basic Principles of Molecular Microbiology

Course Contents

In this lesson, the methods used in molecular microbiology, their application areas and laboratory standardization will be explained

Weekly Schedule

1) Use of molecular methods in microbiology
2) Structure of nucleic acids
3) Nucleic acid Isolation methods
4) Principles ofamplification
5) Types of polymerase chain reaction
6) Real-Time PCR
7) Reverse PCR
8) Mid term exam
9) Flowcytometry
10) Microarray
11) Clonig
12) Cloning
13) Clone Library and sequencing
14) Random shotgun sequencing
15) Next generation sequencing
16) Final exam

Recommended or Required Reading

- Molecular Medical Microbiology Yi-Wei Tang, Max Sussman, Dongyou Liu, Ian Poxton, Joseph Schwartzman
- https://www.google.com/url?sa=t&source=web&rct=j&opi=89978449&url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597138/&ved=2ahUKEwja2dvo8dqGAxWHVfEDHXGdDHUQFnoECBYQAQ&usg=AOvVaw3x2Dz0S2S933xbHFNkHFAN

Planned Learning Activities and Teaching Methods

1) Lecture
2) Question-Answer
3) Discussion
4) Group Study
5) Lab / Workshop


Assessment Methods and Criteria

Contribution of Semester Studies to Course Grade

40%

 

Number

Percentage

Semester Studies

Midterm Examination

1

60%

Quiz

1

20%

Laboratory

1

20%

 

Contribution of Final Examination to Course Grade

60%

Total

100%

Language of Instruction

Turkish

Work Placement(s)

Not Required